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The cells were cultured in RPMI-1640, DMEM, MEM F-12K, or McCoys 5 A medium (Meilunbio, China) supplemented with 10% FBS (Gibco, USA) and 1% antibiotics (Gibco, USA)

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Less-selective compounds in the same class (GHRP-2, GHRP-6, hexarelin) cross-activate other endocrine axes alongside GH, which makes them noisier reference tools when researchers want to isolate ghrelin-pathway signalling from confounding hormonal effects

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