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Key principles include: Maintaining a modest caloric deficit (300-500 calories below maintenance) to prevent rapid weight regain Emphasizing protein intake (1.2-1.6 g/kg body weight) to preserve lean muscle mass and promote satiety, with individualization for those with chronic kidney disease or other conditions requiring protein modification Incorporating high-fiber foods to enhance fullness and support glycemic control Practicing mindful eating and portion awareness to compensate for increased appetite Limiting ultra-processed foods and added sugars that promote overconsumption Physical activity plays a crucial role in weight maintenance and glucose regulation after stopping tirzepatide

Cells were washed in PBS, scraped and suspended in phosphate buffer then sonicated

doi: 10.1056/NEJMoa1012985 196

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