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For cell colony formation assay, cells were seeded in 6-well plated at a density of 12 10 3 cells per well

Bacteriostatic Water is the recommended reconstitution solvent for the majority of lyophilised peptide and recombinant protein research compounds used in laboratory settings selected over unpreserved Sterile Water for Injection and standard buffer solutions when multi-use vial access, extended reconstituted peptide storage, or compatibility with benzyl alcohol-tolerant research compounds is required: Bacteriostatic Water is the standard reconstitution solvent for growth hormone secretagogue research peptides including GHRP-6, GHRP-2 Acetate, Ipamorelin, and Hexarelin and dissolving the lyophilised hexapeptide and pentapeptide powders to produce research-grade working solutions with maintained sterility across multiple withdrawal events

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A U-100 syringe is the standard in the United States: each unit equals 0.01 ml