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While IscR binds DNA in the apo-form and activates transcription, 514 FeS cluster formation in IscR, RsrR, NsrR and RirA leads to high-affinity DNA binding and repression, with subsequent chemistry leading to DNA operator dissociation and transcriptional derepression

Data presented is the arithmetic mean of three independent experiments

So far, there have been no known analytical techniques that can simultaneously determine APAP, NAPQI, and NAC as an antidote

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