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Spheres were then washed once in PBS and imaged in fresh PBS on an Olympus IX83 Inverted Microscope (Mason Technology) using the TRITC (Ex/Em: 396/580 nm) and DAPI (Ex/Em: 353/483) filter sets

Coders cross-checking musculoskeletal diagnosis codes such as M72.8 against payer LCDs benefit from a centralized coding workflow that flags non-compliant pairings before submission