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The role of ferroptosis in regulating normal epidermal differentiation was hypothesized and confirmed by treating an iron chelator to human skin organoids ( Mll4- knockout mice and Alox12b loss-of-function mutant mice exhibited epidermal hyperproliferation, scaling, and other cutaneous manifestations similar to ichthyosis, as well as a deficiency of ALOX12b expression in the epidermis of both mice

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The band intensity was measured using Quantity one software program after normalization with the band density of -actin as negative control