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In db/db mice, CK treatment increases secondary BAs, such as lithocholic acid (LCA) and deoxycholic acid (DCA) through changes in the gut microbiota, especially the Ruminococcaceae family, and up-regulation of cytochrome P450 family 7 subfamily B member 1 ( Cyp7b1 ) and cytochrome P450 family 27 subfamily A member 1 ( Cyp27a1 ) and down-regulation of cytochrome P450 family 8 subfamily B member 1 ( Cyp8b1 ), which changes BA biosynthesis from the classical pathway to the alternative pathway

Within a research context, this compound is studied for its interactions with cell migration , angiogenesis-related pathways , and tissue repair mechanisms

The peptide binds by a two-domain mechanism, with its C-terminus held by the extracellular domain while its N-terminus inserts into the transmembrane bundle to switch the receptor on

Analysis of relative gene expression data using real-time quantitative PCR and the 2 CT method