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injections of 10 nmol kg 1 semaglutide ( n = 8 mice), 100 nmol kg 1 GLP-1MK-801 ( n = 8 mice) or vehicle (isotonic saline, n = 8 mice) at a volume of 5 l g 1

Dose calculations at 5,000 mcg/mL: 1mg dose = 0.2mL = 20 units on U-100 syringe 2mg dose = 0.4mL = 40 units 4mg dose = 0.8mL = 80 units 6mg dose = 1.2mL = 120 units 8mg dose = 1.6mL = 160 units 12mg dose = 2.4mL = 240 units This dilution makes higher doses more practical within a single syringe for mid-range protocols

Intended Use: For Laboratory Research Use Only Key Characteristics of Retatrutide (60 mg) Triple Receptor Agonist (Research Context): Designed to engage GLP-1, GIP, and glucagon receptors in experimental systems

Contrary to its limited role in feeding behavior, ghrelin is a key regulator of glucose homeostasis