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the glutathione present in the elution buffer can disrupt the disulfide bridges present in factor Xa leading to inefficient digestion of fusion protein

Gemeinsamer Bundesausschuss (G-BA): Richtlinie zu Untersuchungs- und Behandlungsmethoden der vertragsrztlichen Versorgung (Richtlinie Methoden vertragsrztliche Versorgung): www.g-ba.de/downloads/62-492-1842/MVV-RL_2019-02-21-iK_2019-04-01.pdf (letzter Zugriff: 17

This study suggests that AMPK has effects beyond autophagy, as it did not link the lifespan extension caused by GR with autophagy as previously indicated