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acute inflammatory conditions, myeloproliferative neoplasms, liver disease)16 or the presence of anti-intrinsic factor antibodies that can cause interference with the B12 assay, resulting in an artefactually high total B12 concentration.17 Active B12 (holotranscobalamin) Active B12 (holotranscobalamin) testing measures the biologically active fraction of B12 available to cells and is particularly useful for assessing potential deficiency in individuals with indeterminate total serum B12 concentrations

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Generation and identification of reactive metabolites by electrochemistry and immobilized enzymes coupled on-line to liquid chromatography/mass spectrometry