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doi:10.3390/nu13041262 Weng Z, Zhang B, Asadi S, et al

Viewing them as alternatives to each other misses the point of why they're combined

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Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0