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Fuentes cientficas Pickart L, Vasquez-Soltero JM, Margolina A

We generated a library of ~340,000 uniquely barcoded transposon mutants in K56-2 (approximately equally divided into 10 subpools)

In this process, the enzymatic hydrolysis occurs concomitantly with the fermentation in the same reaction vessel

All lines of evidence indicate that fast and slow TGR inhibitors bind different conformational states present in the TGR enzymatic cycle induced by both NADPH-dependent reduction and the concomitant destabilization of the polypeptide chain, as shown by TSA