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All the data analysed in this study were collected on the experimental weeks when all five animals were involved in the two-session operant food intake paradigm [34] on each weekday, tested simultaneously in five separate operant chambers (see also Supplementary Methods, Operant chambers)

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Multiple Reaction Monitoring (MRM) is a specific mode within LC-MS/MS, ideal for targeted quantification of acylcarnitines, providing both high sensitivity and specificity by monitoring specific precursor-to-product ion transitions

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