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The assay was designed to demonstrate the biochemical activity of c-met binding sites including Y198 and Y250 (in K1 and K2 domains of HGF, respectively) by significant changes in optical absorbance (expressed as relative unit) within a range from a baseline level (a negative control without HGF

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For quantification of sulfur-containing metabolites in cultured cancer cells and mouse serum, 10 l samples were extracted with 90 l of ice-cold extraction solvent (80% methanol and 20% H 2 O) containing isotope-labelled internal standards (36 M 13 C 2 15 N-GSH, 0.92 M 13 C 4 15 N 2 -GSSG and 10 M D 4 -cystine)

[DOI] [PMC free article] [PubMed] [Google Scholar] 145.Hammond B.R., Jr., Miller L.S., Bello M.O., Lindbergh C.A., Mewborn C., Renzi-Hammond L.M