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Nanocarrier uptake in midbrain organoids Control or PD organoids were embedded in 10 l Geltrex (Thermo Fischer Scientific) at the bottom of 24-well plates (1 organoid/well), then culture inserts were placed above them containing the BBB model as described in our previous study [24]

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In particular, the pivotal biochemical pathways (i.e., apoptosis and oxidative stress, mitochondrial impairment, dysfunctional mitophagy, unfolded protein stress, and improper removal of misfolded proteins) have been widely explored in cell lines, challenged with toxic insults or genetically modified