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Reversible changes in brain glucose metabolism following thyroid function normalization in hyperthyroidism

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

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This premature breakdown can lead to the release of byproducts that irritate the stomach lining, leading to that "pain" or "heavy" feeling