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MichaelisMenten analysis revealed catalytic efficiencies ( k cat / K m ) of 260 mM 1 s 1 , 310 mM 1 s 1 and 190 mM 1 s 1 for D1, D2 and D3, respectively, 1.72.8-fold higher than that of WT BoNT/E protease (110 mM 1 s 1

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