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High-dose IV vitamin C has been used safely for decades and is well-tolerated by most patients

These include individuals with a personal or family history of medullary thyroid carcinoma, those diagnosed with multiple endocrine neoplasia syndrome type 2, patients with severe gastrointestinal conditions such as gastroparesis, individuals who are pregnant or breastfeeding, and patients with severe renal impairment

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Elute with 2 mL of 4% ammonia in methanol solution, and collect the eluate in a 15 mL centrifuge tube