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Fer-1 has been shown to counteract ferroptosis-induced chondrocyte mortality and cartilage deterioration in OA models [104,105]

the present invention is related to a process for producing semapeptide, the process comprising the steps of: a) culturing a host cell comprising a nucleotide sequence encoding of Formula (II) under suitable conditions for expression, insoluble tag is a nucleotide sequence of Alanine -Valine SUBSTITUTE SHEETS (RULE 26) b) recovering semapeptide, wherein semapeptide amino acid sequence is Glu-Gly-Thr-Phe- Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg- Gly-Arg-Gly

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In clinical trials, the dosage has started at 1 mg or 2 mg, then increased every 4 weeks until a maintenance dose of 4 mg, 9 mg, or 12 mg is reached