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The functional medicine investigation of tinnitus often uncovers overlapping drivers: gut dysbiosis and intestinal permeability affecting systemic inflammation, MTHFR and methylation impairment disrupting neurotransmitter synthesis, and cognitive and mood dysregulation amplifying central sensitisation to auditory signals

In the last 2 h of the 24-h permeability assays, the acceptor compartments were supplemented with Hoechst 33342 dye (2 g/ml) to label the cell nuclei of organoids