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Owing to these actions, the principal sites of POMC expression and its processing into biologically active -MSH lie within the central nervous system (hypothalamus and pituitary gland) and in the skin, but there is some evidence demonstrating that -MSH is also produced in other peripheral tissues (Smith and Funder, 1988)

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Dilute aqueous ammonia (1 to 10%): for strongly acidic sequences that need a mildly basic environment

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