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Cantor used static and nanosecond fluorescence polarization techniques to analyze the ribosomal protein S1 fluorescently labeled by iodoacetylethylenediamine (1,5)-napthol sulfonate (IAEDANS) and revealed that this protein is characterized by segmental flexibility, suggesting that it contains at least two domains that can rotate independently (Chu and Cantor, 1979)

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The practical solution: use a smaller reconstitution volume (1.0 mL) during the early titration phase so the total solution volume is smaller and you draw more per dose (relatively), or accept that you will waste some peptide during the titration stages

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