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298 ApoMT (metal-free MT) has been identified as a potential agent for extracting zinc from NF-B, thereby rendering the NF-B-mediated transcriptional activity inactive due to zinc chelation

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Written informed consent was waived, since no specific intervention related to the research was performed

Briefly, 300,000 to 400,000 HeLa or U2OS cells were transiently cotransfected with precomplexed RNPs consisting of 100 to 150 pmol of chemically modified sgRNA (Synthego), 33 to 50 pmol of 3 NLS Sp Cas9 protein (St