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This might be a result of the low HLB values of both lipids forming the lipid phase enabling a comparatively low water uptake

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To stain nuclei of the neurons, the specimens from NG were incubated with Alexa Fluor 488-conjugated Anti-NeuN IgG (Supplementary Table S1) at RT for 1 h, and the specimens from NTS and ARC were incubated overnight with Alexa Fluor 555-conjugated anti-NeuN IgG (Supplementary Table S1) at 4 C