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Summary Keywords glutathione, pyocyanin, oxidative stress, biofilm, P

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2 Emergency Trauma Center, Nanyang Second General Hospital, Nanyang, Peoples Republic of China *These authors contributed equally to this work Correspondence: Guixin Sun, Department of Traumatic Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, Peoples Republic of China, Tel +21-38804518, Fax +21-65983047, Email [email protected] Background: Oxidative stress status may affect bone metabolism and regeneration

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Quantified proteins using Bradford assay (Bio-Rad) using BSA as a standard

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