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Bergenstal RM, Wysham C, Macconell L, DURATION-2 Study Group, et al

Journal of Nutrition and Metabolism

More commonly, they can also cause nausea, vomiting, migraines and disturb sleep, which can turn some patients off the drugs

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes