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In order to evaluate the mechanisms of action of tirzipatide, in a recent study [62] subjects with T2DM performed a deep metabolic evaluation with measures of beta cell function and insulin sensitivity by hyperglycemic clamp, mixed meal test and euglycemic clamp before and 28 weeks after treatment with tirzepatide 15 mg or semaglutide 1 mg

The stranded mRNA-seq library was generated from 1000 ng of total RNA from each sample using Truseq stranded mRNA library kit (Illumina) according to the product instructions

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