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Int J Mol Sci (2019) 20:1475

Using the regenerative power of your own blood, platelet-rich fibrin (PRF) microneedling harnesses the growth factors in your platelets to increase the collagen induction benefits of microneedling

Cell culture Human lung carcinoma A549 cells, mouse fibroblasts 3T3-L1, human lung adenocarcinoma cell line NCl-H441 (ATCC, LGC Standards S.a.r.l, Molsheim, France) and 13s24 rat olfactory neuroblasts (Coronas et al, 1997) were grown in Dulbeccos Modified Eagles Medium with low glucose (1 g/L) containing 10% fetal calf serum (DMEM 10% FCS, PAA Laboratories, Canada), streptomycin plus penicillin (100 units/mL