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One hour later, islets were centrifuged for 3 min at 400 g , supernatant was conserved for secreted insulin determination and total insulin was extracted using acidified ethanol (0.135 M HCl in 75% ethanol)

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PMID: 41327798 Eur J Pharmacol Involvement of glucagon-like peptide-1 receptor in fisetin tetramethyl ether-enhanced insulin secretion in MIN6 cells

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