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Samples were separated on SDSPAGE gels, transferred to PVDF membranes and blocked in 2% fatty acid-free BSA and 5% skim milk powder in PBS containing 0.2% Tween-20 (PBST) for lipoate detection, or 5% skim milk powder in PBST for glutaryl-lysine detection

The relative p70S6K1 or p70S6K2 mRNA levels were calculated by the 2 Ct method

The aggregates participate in initiating processes in which molecules of p53 widely unfold and then aggregate, resulting in the emergence of amyloid-containing structures

When taken as a liquid, glutathione is absorbed through mucous membranes which quickly delivers a high-dosage of glutathione directly to the bloodstream