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Monitoring the effluent at 220 nm, chromatography (flow rate: 1.0 mL/min) was started with a mixture of H 2 O/MeOH (40/60, v/v), the MeOH content was kept constant for 1 min at 60%, increased within 20 min to 80%, increased within 5 min to 86%, increased within 10 min to 90%, increased within 1 min to 100%, kept constant for 3 min, decreased within 1 min to 60% MeOH, followed by re-equilibration for 4 min

Tirzepatide is a dual agonist, meaning it activates two hormone pathways: glucose-dependent insulinotropic polypeptide, or GIP, and glucagon-like peptide-1, or GLP-1

Collectively, these findings substantiate kaempferol's therapeutic promise as a safe, multifactorial candidate for both prevention and treatment of Alzheimer's disease by targeting oxidative stress, neuroinflammation, amyloid pathology, and neuronal dysfunction

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