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Materials and methods DF-1 cells and IBDV culture Routine cell culture of adherent DF-1 cells was performed in Nunc EasyFlask 25 cm 2 (Thermo Scientific) with 5 ml DMEM/F12 (1:1) with 5% fetal bovine serum (Biological Industries) in a humidified incubator at 37 with 5% CO 2

For Ifng , Gzmb , Prf1 and Ly6G mRNA determination primer sequences are available upon request

Designed strictly for laboratory use, it is a dependable, high-standard reference material for any qualified research facility mapping the melanocortin system