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BPC-157 and TB-500 Dosage Protocol BPC-157: 250500mcg daily, injected subcutaneously near the injury site TB-500: 22.5mg twice weekly (loading phase) for 46 weeks, then 2mg once weekly (maintenance phase) The combination is typically used for 612 weeks, depending on injury severity and healing progress

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After 24 h, cells were expanded into six-well dishes and selected in 1 g ml 1 puromycin for 3 days, then further expanded in nonselective medium