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Results SARS-CoV-2 recombinant S1 protein induces endothelial inflammation and cell damage independently of viral infection To study the global effect of SARS-CoV-2 spike protein on endothelial cell protein expression, we assessed the proteome of hMEC after 24 h incubation with rS1p
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this leads to increased genetic instability and heightened activity of DNA repair mechanisms, such as overexpression of poly (ADP-ribose) polymerase
Desalting of the samples was performed online using a reversed-phase Symmetry C18 trapping column (180 m internal diameter, 20 mm length, 5 m particle size, Waters)
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