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The absorbance was measured at 540 nm using an Epoch Microplate Spectrophotometer (BioTek Instruments Inc., Winooski, VT, USA)

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2.2.2 ERK1/2-HDAC6 signaling pathway The GLP-1R-ERK1/2-HDAC6 signaling pathway complements the mitophagy pathway by modulating oxidative stress and autophagy

The discovery that the prostaglandin PGE 2 , a compound normally associated with inflammation and fever, is a potent regulator of normal male development was not only a surprise, but it also illustrated the point of how little we know about brain development and how comparing and contrasting the male and female brain at this highly dynamic time point can provide novel insights into previously unconsidered mechanisms