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The pharmacokinetics of getting an endogenous peptide to behave usefully as a therapeutic, half-life, route of administration, immunogenicity, tachyphylaxis, are non-trivial engineering problems that have killed many promising candidates

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N-ethylmaleimide is frequently used to trap free thiols, but reagent choice, timing, collection tubes, anticoagulants, storage conditions, and freeze-thaw limits must be validated for the matrix and analytical method

(PubMed) Hardwick RN, Clarke JD, Lake AD, et al