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The samples were processed in a series of ethanol grades, then underwent xylene clearing, infiltration, and embedding in Paraplast tissue embedding media (Leica biosystems)

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These results emphasize that GPX4 is required in Treg cells in order to prevent ferroptosis to maintain normal Treg cell function and sustain immune homeostasis ( Previous research results supported that activation of GPX4 contributes to the suppression of inflammation ( Nrf2 downstream target genes was increased in psoriasis lesions by comparison with healthy and uninvolved skin samples ( UV radiation (UVR) exposure can activate the cutaneous immune system by releasing pro-inflammatory factors, ROS, and inducing apoptosis in keratinocytes, thereby causing sterile inflammation or cancer ( Feng et al