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In total, 300 islets from both experimental groups were frozen at 80C and subjected to posterior sonication (Sonic Dismembrator Model 100, Thermo Fisher Scientific, Waltham, MA, USA) in lysis buffer (in mmol l-1: HEPES, 50

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At the same time, IL-6 and TNF- up-regulate hepatic CRP, and elevated CRP can further stimulate the release of inflammatory mediators, such as IL-6 and TNF-, to exert an inflammatory effect

4D), we did not observe an increase in p21 expression upon treatment with MQ, the active metabolite of APR-246 (Supplementary Fig