glutathione affinity chromatography

glutathione affinity chromatography Purification using Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B Columns can be used as a spin format of gravity flow columns glutathione in affinity chromatography Schematic

Name: glutathione affinity chromatography Purification using Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B Columns can be used as a spin format of gravity flow columns glutathione in affinity chromatography Schematic
Brand: www.rasophiles.fr
SKU: 32625048418
Price: 29.84 USD
Availability: InStock
Rating: 4.4734 (29 reviews)

SKU: 32625048418

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Description

J.KahlerC

Sequential washes were performed twice for each wash buffer: A (20 mM TrisHCl pH 8.0, 2 mM EDTA pH 8.0, 1% Triton X-100, 150 mM NaCl, 0.1% SDS, 1 mM PMSF), B (20 mM TrisHCl pH 8.0, 2 mM EDTA pH 8.0, 1% Triton X-100, 500 mM NaCl, 0.1% SDS, 1 mM PMSF), C (10 mM TrisHCl pH 8.0, 2 mM EDTA pH 8.0, 250 mM LiCl, 0.5% NP-40, 0.5% deoxycholate), and TE buffer