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Adoptive transfer As described 52 , splenocytes were collected by gently pushing spleens through a 100-m strainer, and red blood cells were lysed with ACK lysing buffer (Gibco, A10492-01)

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Frozen cortical samples (the weights of which were determined beforehand) were homogenized in 5% 5-sulfosalicylic acid (SSA), and the mixtures were centrifuged at 8000 g for 10 min at 4 C