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Membranes were then incubated for 30 min at RT in HRP-conjugated secondary antibody (Santa Cruz Biotechnology, Inc, USA) diluted in 5% milk or BSA in TBS-T

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The production of A was effectively reduced in Fru + amlodipine (33%), Fru + aminoguanidine (37%), and Fru + NAC (19%) versus Fru