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An additional volume of blood was collected to measure liver enzymes and basic metabolic parameters using an automated chemistry analyzer (data not shown, no significant difference between groups or time points)

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Patent US5164303

The fluorescence spectrum of the lysate of Halo-Rac1-expressing cells, subtracted by that of the non-transfected cells, and the fluorescence spectrum of the SF650T ligand subtracted by that of the sonication buffer alone is shown in Supplementary Fig