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The human GCLC expression plasmid was constructed using the Gateway Cloning system (Thermo Fisher Scientific) and the pCMV-SPORT6-GCLC vector containing a human GCLC cDNA clone (Horizon Discovery Catalog #: MHS6278-202759380, Clone ID: 6052162, NCBI GeneBank Accession: BC039894), the Gateway pDONR221 Vector (Thermo Fisher Scientific), and the pLenti-CMV-Neo-DEST (Addgene Plasmid #17392) destination vector

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In the landmark STEP 1 trial (Once-Weekly Semaglutide in Adults with Overweight or Obesity, Wilding and colleagues, New England Journal of Medicine , 2021), 1,961 adults who took weekly semaglutide (2.4 mg) plus lifestyle support lost a mean of 14.9% of their body weight over 68 weeks, compared with 2.4% on placebo, and 86% achieved at least 5% weight loss

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