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Description

For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)

Cancer 144 (7), 15301539

In the control medium, the callus exhibited a friable texture and a greenish/yellowish-brown color, along with profuse proliferation and having biomass of 348723.53 mg FW and 12813.76 mg DW