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The cells were mechanically crushed by using a cell scraper in 2 mL of methanol after six times PBS washing (2 mL each)

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Studies in Caco-2 cell monolayers (a cellular model of human intestinal epithelium) have shown that metformin is efficiently taken up across the apical (luminal-facing) surface of enterocytes via bidirectional transporters, but that efflux across the basolateral surface of enterocytes is limited, resulting in the accumulation of metformin in the epithelium [16], possibly accounting for the greatly increased metformin concentration seen in these cells

Understanding Glutathione and Resveratrol What is Glutathione