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Supernatant was removed, pellets were lysed in 70 L Cell Culture Lysis Reagent (E1500, Luciferase Assay System, Promega, Walldorf, Germany) on ice for 15 min and cleared by centrifuge at 8000 g and 4 C for 5 min

doi: 10.1159/000017332 98 SimpsonIACarruthersAVannucciSJ

[1] [5] Orthostatic hypotension has been reported with Saxenda use and may occur particularly in patients taking antihypertensive medications or those with autonomic dysfunction