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The 6 to 25% gradients were centrifuged at 39,000 rpm for 16 hr in a SW41 rotor (Beckman) for the separation of the 5S/14S intermediates

Diabetes Care 30:523528 de Zeeuw D, Akizawa T, Agarwal R, Audhya P, Bakris GL, Chin M, Krauth M, Lambers Heerspink HJ, Meyer CJ, McMurray JJ, Parving HH, Pergola PE, Remuzzi G, Toto RD, Vaziri ND, Wanner C, Warnock DG, Wittes J, Chertow GM (2013) Rationale and trial design of bardoxolone methyl evaluation in patients with chronic kidney disease and type 2 diabetes: the occurrence of renal events (BEACON)

Each vial pairs three signaling molecules studied for distinct repair steps

The reduction of parasympathetic tone can undergo tachyphylaxis with chronic GLP-1 stimulation