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Samples were imaged at room temperature in a humidified imaging chamber with a Definite Focus module (Zeiss) employed for thermal drift correction and ZEN Blue v3.2 (Zeiss) software used for acquisition and deconvolution (LSMPlus function)

Interestingly, GLP-1 and its analogue exendin-4 have been shown in mouse models to reduce levels of amyloid-beta in the brain as well as the beta-amyloid precursor protein found in neurons