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Piezo2, expressed in TRPV1-lineage nociceptors innervating the colon, contributes significantly to visceral mechanosensitive and nociception under both normal and pathological conditions [99,100,101] Additionally, TRPV1-expressed visceral neurons play a crucial role in chronic visceral pain through microglial activation in the spinal cord, mediated by purinergic signaling involving neuronal ATP and microglial P2Y12 receptors [102]