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It can be measured in several ways: Sets and Repetitions (Reps): A set is a group of consecutive repetitions

Vial Size by Protocol Tolerance testing: A 2mg or 5mg vial is the smallest unit cited 1-3 weeks at standard doses Standard 200mcg/day pre-bedtime protocol: 5mg vial 25 days, fits the stability window The most-cited size in vendor sales data 1-2 vials per month Extended cycles: 10mg 5 kit drops cost to $4.40-10/mg Each vial reconstituted only when needed

Finally, the pictures were captured using a Leica DM4000 B epifluorescence microscope (Leica Microsystems, Wetzlar, Germany), and the relative fluorescence intensity was calculated using Image-Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, USA)